Package Description

Dental unit water line testing for microbial contamination is a major issue in dentistry; several outbreaks have occurred due to the failure of dental offices to effectively test and remediate their dental unit water lines. These outbreaks pose a potentially significant risk to children and immunocompromised patients. Flow Cytometry offers an alternative to culture based methods for counting bacteria that offers a rapid and straightforward solution to enhance health outcomes in dental offices and improve upon current paradigms. Culture based assays, such as R2A, which is the current standard for dental unit waterline testing are inherently limited as only a portion of microorganisms utilize the nutrients and are recovered on agar media. Flow cytometry is a well-established technology that can be adapted to enhance testing speed and sensitivity outcomes. Research demonstrates that FCM methods shorten the length of time between testing and actionable results, which can support clinicians ability to limit patient exposure to potentially harmful bacteria This presentation will share the results of research comparing the current R2A standard and flow cytometry metrics are directly compared, and an empirical threshold will be presented to align results between the two technologies. Practical Implications: This presentation will show how FCM offers advantages over HPC methods for monitoring DUWL contamination that is more accurate and timely in providing water monitoring results for practitioners.

LEARNING OBJECTIVES

  1. Describe how flow cytometry (FCM) technology works, and how it differs from other methods for measuring micro-organisms in water
  2. Describe the different measures provided by FCM and their significance in evaluating the microbiological quality of dental procedural water
  3. Describe how FCM can potentially augment or replace culture methods for microbiological enumeration in dental procedural water

Price:

  • Members: $15
  • Non-members: $30

Continuing Education

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ADS is an ADA CERP Recognized Provider.

ADA CERP is a service of the American Dental Association to assist dental professionals in identifying quality providers of continuing dental education. ADA CERP does not approve or endorse individual courses or instructors, nor does it imply acceptance of credit hours by boards of dentistry.

Concerns or complaints about a CE provider may be directed to the provider or to the Commission for Continuing Education Provider Recognition at ADA.org/CERP.

Please email the ADS office at [email protected] or call +1 (410) 571-0003 if you wish to be in contact with the course author/creator(s) with any questions or for clarification of course concepts or for technical assistance in completing the online test.

All participants assume individual responsibility for providing evidence of contact hours of continuing education to the appropriate authorities and for the maintenance of their individual records.

Learn more about ADS’s CE Program & Provider Recognition.

CE Credits: 1.0
Educational Method: Lecture, Recorded, Self-instructional (self-study)
Location: Online
Publication Date: June 2025
Expiration Date: June 30, 2028

Speakers:

Clifton Carey, PhD

Education, Licensure & Certifications

  • PhD, Analytical Chemistry, American University
  • MS, Biology, East Carolina University
  • BA, Chemistry & Biology, St. Andrews Presbyterian College

Affiliations

  • Academy of Operative Dentistry
  • American Association of Dental Research (AADR)
  • American Chemical Society (ACS)
  • European Organization for Caries Research (ORCA)
  • International Association of Dental Research (IADR)
  • Washington DC Professional Chapter of Alpha Chi Sigma (AXE)

Leadership Roles:

  • President, Colorado Section of AADR 2014 - present
  • ISO TC106 – Dentistry:
    •Vice Chair of the US delegation on Dental Products (SC7)
    •Convener of the Work Group for Analysis of Oral Care Products
    •US expert on:
    •Toothpaste
    •Oral Rinses
    •Denture Adhesives
    •Fluoride Varnishes
    •Dental Bleaching
  • ANSI Chairman: bleaching, fluoride analysis, toothpastes, oral rinses, denture adhesives, and fluoride varnishes
  • Counselor, Washington DC Section of AADR 2001 - 2011
  • Board of Editors Journal of Research NIST 2000 - 2011
  • President Washington DC section of the AADR 2000 - 2002
  • President, Alpha Chi Sigma Beta Upsilon Chapter 1988-1990

Disclosure: No relevant financial relationships to disclose

Shannon Mills, DDS

Dr. Mills is a retired Air Force Dental Officer, dental educator, and dental benefits executive.  He is currently an independent consultant, lecturer, and author and is internationally recognized as an expert in infection prevention and control. He has been a member of the Association for Dental Safety (ADS) since 1991 and is a recipient of the ADS James J. Crawford Award for Lifetime Achievement. He represents ADS as a volunteer member of the American National Standards Institute/American Dental Association Standards Committee.  Until the untimely elimination of the CDC Division of Oral Health in April 2025, he served as a contract subject matter expert supporting the Healthcare Infection Control Practices Advisory Committee (HICPAC) on dental water quality. 

Disclosures: Contract for advisory services with ProEdge Dental Products

Refund & Cancellation

This course is non-refundable. Click here to view the complete Product Agreement.